Preparing a Glucose Solution: A Step-by-Step Guide Using 0.125 g of Glucose

In the preparation of solutions, precise measurements are crucial. For instance, using 0.125 g of glucose to prepare a solution is significant because it allows for accurate control over the concentration, ensuring consistency and reliability in experiments or applications. This specific amount is often used in scientific research to achieve desired solution properties.

Materials and Methods

Materials

1. Glucose: Use analytical grade D-glucose.
2. Solvent: Distilled water.
3. Equipment:
   • Analytical balance
   • Weighing paper or boat
   • Beaker (100 mL)
   • Stirring rod
   • Graduated cylinder (100 mL)
   • Volumetric flask (100 mL)

Methods

1. Weighing the Glucose:

   • Place a piece of weighing paper or a weighing boat on the analytical balance.
   • Tare the balance to zero.
   • Accurately weigh 0.125 g of D-glucose.

2. Dissolving the Glucose:

   • Transfer the weighed glucose into a 100 mL beaker.
   • Add approximately 50 mL of distilled water to the beaker.
   • Stir the mixture with a stirring rod until the glucose is completely dissolved.

3. Transferring to Volumetric Flask:

   • Pour the glucose solution into a 100 mL volumetric flask using a funnel if necessary.
   • Rinse the beaker with distilled water and add the rinsing to the volumetric flask to ensure all glucose is transferred.

4. Adjusting the Volume:

   • Add distilled water to the volumetric flask until the bottom of the meniscus is at the 100 mL mark.
   • Cap the flask and invert it several times to ensure thorough mixing.

This method ensures an accurate and consistent preparation of the glucose solution.

Procedure

Here’s a step-by-step procedure for preparing a solution using 0.125 g of glucose:

1. Weigh the Glucose: Use a precise balance to measure 0.125 g of glucose.
2. Prepare the Container: Take a clean, dry container (like a beaker or flask).
3. Add Glucose to Container: Transfer the 0.125 g of glucose into the container.
4. Add Water: Measure a specific volume of distilled water using a graduated cylinder. Add this water to the container with the glucose.
5. Dissolve the Glucose: Stir the mixture with a glass rod or use a magnetic stirrer until the glucose is completely dissolved.
6. Adjust Volume: If necessary, add more distilled water to reach the desired final volume of the solution.
7. Label the Solution: Clearly label the container with the concentration and contents of the solution.

And that’s it! Your glucose solution is ready to use.

Calculations

Here are the calculations:

1. Concentration (ppm):

   • Convert 0.125 g of glucose to mg:

     $$0.125 \, \text{g} \times 1000 \, \text{mg/g} = 125 \, \text{mg}$$0.125g×1000mg/g=125mg

   • Assume the total mass of the solution is 250 g (for example).
   • Convert 250 g to kg:

     $$250 \, \text{g} \times \frac{1 \, \text{kg}}{1000 \, \text{g}} = 0.25 \, \text{kg}$$250g×1000g1kg​=0.25kg

   • Calculate ppm:

     $$\text{ppm} = \frac{125 \, \text{mg}}{0.25 \, \text{kg}} = 500 \, \text{ppm}$$ppm=0.25kg125mg​=500ppm

2. Molarity (M):

   • Molar mass of glucose (C₆H₁₂O₆):

     $$6 \times 12.01 + 12 \times 1.01 + 6 \times 16.00 = 180.16 \, \text{g/mol}$$6×12.01+12×1.01+6×16.00=180.16g/mol

   • Convert 0.125 g of glucose to moles:

     $$\frac{0.125 \, \text{g}}{180.16 \, \text{g/mol}} = 0.000694 \, \text{mol}$$180.16g/mol0.125g​=0.000694mol

   • Assume the volume of the solution is 250 mL (0.250 L).
   • Calculate molarity:

     $$\text{M} = \frac{0.000694 \, \text{mol}}{0.250 \, \text{L}} = 0.00278 \, \text{M}$$M=0.250L0.000694mol​=0.00278M

Applications

A solution prepared with 0.125 g of glucose can have several applications across different fields:

1. Biotechnology: Glucose solutions are often used as a carbon source in microbial culture media. This small amount can be crucial for experiments involving the growth of bacteria or yeast, especially in controlled lab settings.

2. Biochemistry: In enzymatic assays, glucose can act as a substrate to study enzyme kinetics. For example, glucose oxidase assays use glucose to measure enzyme activity, which is important in diabetes research and diagnostics.

3. Pharmaceuticals: Glucose solutions are used in drug formulation and delivery. They can serve as stabilizers for certain medications or as a component in intravenous solutions to provide energy to patients.

4. Food Industry: Glucose is a key ingredient in fermentation processes, such as in the production of alcohol, vinegar, and certain dairy products. Even small amounts can influence the flavor and texture of food products.

5. Chemical Industry: Glucose can be used in the synthesis of biobased surfactants and other chemicals. These surfactants are important in creating eco-friendly cleaning products and personal care items.

6. Environmental Science: Glucose solutions can be used in bioremediation to stimulate the growth of microorganisms that degrade environmental pollutants.

Each of these applications highlights the versatility and importance of glucose in scientific and industrial contexts.

A Solution Prepared with Glucose: Applications Across Different Fields

A solution prepared with 0.125 g of glucose can have various applications across different fields due to its versatility and importance in scientific and industrial contexts.

To prepare this solution, the first step is to calculate the number of moles of glucose present in 0.125 g using the molar mass of glucose (180.16 g/mol). This yields approximately 6.97 x 10^-4 mol.

Next, assuming a volume of 250 mL (0.250 L) for the solution, the molarity can be calculated as 0.00278 M. This concentration is crucial in various applications, including biotechnology, biochemistry, pharmaceuticals, food industry, chemical industry, and environmental science.

In these fields, glucose solutions are used as a carbon source in microbial culture media, act as substrates to study enzyme kinetics, serve as stabilizers for medications or components in intravenous solutions, influence the flavor and texture of food products, facilitate the synthesis of biobased surfactants, and stimulate the growth of microorganisms that degrade environmental pollutants.

Each application highlights the significance of understanding how a solution is prepared using 0.125 g of glucose, demonstrating its importance in various scientific and industrial contexts.